Does the Portable Penetrator WiFi Cracking software only work with Alfa WiFi Adapter antenna install the vm player on a windows 10 64k machine automatically or do I need to do a separate install?

It works with any supported chipset and includes many different WiFi brands including and not limited to Alfa, TP-Link, Realtek and more.

When you install VMware Player or Workstation on Windows 10 the drivers will automatically be loaded.